![]() ![]() In addition, there were evident clinical features of H. Gram-negative bacilli were seen in the WBC. ![]() Previous population-based studies in Hefei and Nanning, China, found that the incidence of Hib meningitis was 10.66 and 0.98 cases/100,000 children 25 white blood cells (WBC) in each low-power field of view to avoid the collection of colonized H. In recent years, Hib infections have become rare with the widespread introduction of the Hib vaccine. Therefore, simple serotyping will be misjudged as NTHi, and capsular gene testing is required. In a few cases, the encapsulated strains ceased producing capsules to adapt to the environment. NTHi is non-encapsulated and does not agglutinate with either type of serum. There are six serotypes: a, b, c, d, e, and f, often abbreviated as Hia, Hib, Hic, Hid, Hie, and Hif, respectively. influenzae is divided into two types: encapsulated type and non-encapsulated type. influenzae disease has a better prognosis however, the disease burden remains high owing to its high occurrence in children. It is more often severe, with a high mortality rate or various sequelae left in survivors. It can also occur in individuals without any underlying diseases. Children under five years of age, especially those under one year of age, are more likely to develop invasive H. influenzae infection isolated from sterile sites such as blood, pleural fluid, peritoneal fluid, cerebrospinal fluid etc. influenzae, a gram-negative bacillus that commonly colonizes the nasopharynx of humans, is an opportunistic pathogen that can cause upper respiratory tract infection, pneumonia, asthma, other respiratory diseases, and severe systemic infections such as purulent meningitis and sepsis. Keywords: group III-like mutations, BLPACR, prevalence, invasive Haemophilus influenzae, genome influenzae belonging to Hib and ST95 was first reported to cause sepsis in China. Notably, group III-like mutations had increased prevalence among BLPACR strains. ![]() influenzae NTHi with β-lactamase-positive was highly prevalent. influenzae strain 108 revealed regions I–III exist in duplicate however, complete deletion of IS1016 was only present in one of the copies.Ĭonclusion: Non-invasive H. Three prophages were identified, and the capb loci of the H. It belonged to sequence typing ST95 and exhibited sensitivity to all 11 antibiotics. influenzae 108 isolated from blood in China belonged to H. influenzae strains all belonged to non-typeable H. The novel Asn-526→His mutation was present in one β-lactamase-negative AMP-susceptible (BLNAS) strain. Group III-like mutation accounted for 71.15% of β-lactamase-positive, AMC-resistant (BLPACR) strain mutants. The β-lactamase gene of 106 AMP-resistant strains was bla TEM-1. influenzae were as high as 99.06% (after adjustment) and 49.53%, respectively. Results: We observed that the ampicillin (AMP) and amoxicillin/clavulanate (AMC) resistance rates of non-invasive H. Lastly, whole genome sequencing (WGS) was conducted using Illumina HiSeq and PacBio sequencing technology. β-lactamase-producing and serotyping genes were evaluated via multiplex polymerase chain reaction (PCR), and ftsI was amplified using high-fidelity PCR. Methods: Antibiotic susceptibility was tested using the turbidimetric method. influenzae isolated from blood in pediatric patients. influenzae) from pneumonia patients and analyze the whole genome of one invasive H. Purpose: We aimed to evaluate antibiotic resistance and molecular epidemiological characteristics of non-invasive Haemophilus influenzae ( H. Jiansheng Lin, 1, 2 Yinna Wang, 2 Chunli Lin, 2 Ran Li, 3 Gaoxiong Wang 1, 4ġSchool of Public Health of Fujian Medical University, Fuzhou, People’s Republic of China 2Microbiology Laboratory, Quanzhou Women’s and Children’s Hospital, Quanzhou, People’s Republic of China 3Department of Anesthesiology, The Second Affiliated Hospital of Fujian Medical University, Quanzhou, People’s Republic of China 4Research Administration Office, Quanzhou Women’s and Children’s Hospital, Quanzhou, People’s Republic of ChinaĬorrespondence: Ran Li, Department of Anesthesiology, The Second Affiliated Hospital of Fujian Medical University, 34 Zhongshan North Road, Licheng District, Quanzhou, Fujian Province, 350122, People’s Republic of China, Tel +1140, Email Gaoxiong Wang, Research Administration Office, Quanzhou Women’s and Children’s Hospital, 700 Fengze Street, Fengze District, Quanzhou, Fujian Province, 350122, People’s Republic of China, Tel +1685, Email ![]()
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